Please find the following media preparation method:
Mayer's Hematoxylin Solution
Chemicals Amount Function
Aluminum potassium sulphate (alum) 50 g mordant
Distilled water 1000 ml solvent
Hematoxylin 1 g dye
Sodium iodate 0.2 g oxidizing agent
Glacial acetic acid 20 ml pH control
Dissolve alum in distilled water. When alum is completely dissolved, add hematoxylin. When hematoxylin is completely dissolved, add sodium iodate and acetic acid. Bring to boil and cool. Filter if it is necessary.
Staining Characteristic - Progressive.
Immunohistochemistry counterstain for 30 seconds.
H&E stain for 5 minutes.
This recipe gives clear and sharp nucleus staining with little background. Good for both immunohistochemistry counterstain and H&E. This solution is also alcohol and mercury free so it is good for IHC counterstain when AEC substrate is used.