I've been doing my first Western Blotting these past couple of days. I'm studying some muscle proteins.
I've finish the step of runnig the gel and transferring the proteins to the n/c membrane.
However, the gel (in the sandwich) is still in the tank covered in transfer buffer.
I'm going to need to leave for about 5 ays and come back again later to finish with the western.
My question: can I leave it there inside the buffer all that time?
I'm afraid it would be ruined?!
I'd also love to know of all the ways SDS-PAGE gels can be preserved, for how long, and at what step can that happen?
PS: I've made a mistake in my pre-first attempt to do Western; I finished running the gel (electrophoresis) and I had to leave it in the buffer for 2 days (outside of the fridge!), later when I was trying to take it out to lay it on the n/c membrane in the sandwich for the next phase (transferring) it was so fragile that it tear apart very easily!
I'm afraid of doing a similar mistake!