I have a question: can NaN3 ( Sodium Azide) used as conservant for preservimg antibodies influence the reaction between antibody and antigen and what could be a mechanism of such an influence?
Thanks in advance!
Azide prevents cells from using ATP to get things done. In some cases binding of antibodies to antigen may cause the cell to aggregate and internalize the antigen. CD3 is a classic example of antigen capping, so called because of the way the process looks with fluorescence microscopy. This process requires energy and is prevented by the use of azide and/or cold (4°C).
I'm not aware of any direct effect of azide on the actual affinity of the antibody for the antigen. Unless the epitope undergoes some energy-requiring conformational change - haven't heard of such a thing.
thank you for the information
i hope it wont that is why antibody is storing in nan3 if it interfere with ag how they will suggest for storage
But it is possible that the storage is OK (without bakteria ets) but further use is not
The amount of azide used to prevent bacterial growth is very unlikely to interfere with any downstream use of the antibody. Usually the antibody will be highly diluted and/or immobilized and washed before it is used.