We have recently developed a hight throughput windows based toolkit that utilizes sequence similarity to define functional annotations. This software assigns Gene Ontology, Enzyme Commission and KEGG pathways to sequence data utilizing a custom curated database based upon GOA. It can be downloaded for free at our website along with the formatted database. We also have a user freindly distributed BLAST application known as W.ND BLAST (windy BLAST) also available free. It has complete gui's for database engineering, blast engines, as well as a very nice output viewer, curator, annotation engine.