Axoporator 800A

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Fraser Moss
Fraser Moss's picture
Axoporator 800A

Are there any other users of the Axoporator 800A (Axon instruments/Moleculardevices) out there apart from myself and the Rae group who have any tips for success?

In particular - what's the largest plasmid you've managed to transfect?

What is the maximium concentration of nucelic acid anyone has gone up to before it becomes toxic or transfection has failed?