Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells?
The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for.
I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes.
I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse.
If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)