Blocking Phosphatase

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las
las's picture
Blocking Phosphatase

Hi , How can I block phosphatase in a Ser/Thr Protein Inhibitor
Cocktail.
Please respond with suggestion or references.
Thank You

samm
samm's picture
We use the Sigma Phosphatase

We use the Sigma Phosphatase inhibitor cocktail I + II - works fine for Stat6.
For specific targeting of phosphatases, try micysten from Upstate.

COHscientist
COHscientist's picture
How about 50mM NaF, 0.2mM

How about 50mM NaF, 0.2mM Na3VO4? Some literature recommend these two. And In my case it worked fine.

saswati1
saswati1's picture
I use 50 uM (final

I use 50 uM (final concentration) sodium floride in addition to the inhibitor cocktail mixture

las wrote:

Hi , How can I block phosphatase in a Ser/Thr Protein Inhibitor
Cocktail.
Please respond with suggestion or references.
Thank You
Tracy
Tracy's picture
COHscientist wrote:How about

COHscientist wrote:

How about 50mM NaF, 0.2mM Na3VO4? Some literature recommend these two. And In my case it worked fine.

NaF: Ser/Thr Phosphotase inhibitor
Na3VO4: Tyrosine phosphotase inhibitor