I need a good blocking buffer for my chemiluminescent EMSA. The kit I started with will not tell me what is uses in the blocking buffer. I no longer need the kit except for the blocking buffer, and $300.00 per 500 mls is alot to pay. I am using biotinylated target, running it out on a native 7.5% Acrylamide gel and transfering it to a positively charged nylon membrane and then using a SA-HRP conjugate, and developing with a chemiluminescent substrate. All is working well, but I need a good blocking buffer.