Isolation of DNA bands from PAGE

3 posts / 0 new
Last post
Firoz Ahmad
Firoz Ahmad's picture
Isolation of DNA bands from PAGE

Hello!
I want to know is there any kit available for elution of DNA bands from Polyacrylamide gels???
I have used Qaigen and Sigma gel extraction kit but i am not getting good recovery, consequently my down stream processing is hindred.
Any new protocol for elution of DNA bands from Polyacrylamide gels is also welcome!!
Looking forward to hear from you all at yr earliest.
Regards,
FA

Jason King
Jason King's picture
I used to elute protein from

I used to elute protein from PAGE gels and one of the methods I used included a first step in which the gel slice was cut up into small pieces and put in to an eppenforf tube with 200-300L of buffer. The protein moved out of the gel, into the buffer.

As you know when using agarose gels, DNA (especially short molecules) tend to difuse out if you switch off the power supply. Maybe you could elute it in a similar way - into water or TE. I guess it would be more efficient if you used a PAGE gel that was low percentage with little cross-linking.

Firoz Ahmad
Firoz Ahmad's picture
Thank you very much for such

Thank you very much for such a prompt reply. I will work on yr suggestion by using low gel %.
Thanks once again
Firoz