i want to know complete online reference for pulse field gel electrohoresis. Please tell me if anybody knows about any online material or any website dedicated to this technique which has protocols in details?
Find below some good online references for pulsed field gel electrophoresis:
Pulsed Field Electrophoresis for Separation of Large DNA -
MRI Mycobacteria Pulsed-Field Gel Electrophoresis Database contiaining a Standard Operating Procedure For PFGE Of Mycobacterium Avium Subsp. Partuberculosis:http://www.mri.sari.ac.uk/bacteriology-current-project-01-printable.asp#sop
Here is a reference of PFGE protocol for typing bacteria such as E. coli:
Gautom RK. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day.
J Clin Microbiol. 1997 Nov;35(11):2977-80.