Pico Green

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KWaraska's picture
Pico Green

I am reading pico green reactions on a Tecan GENios and can generate the flourescence numbers. How do I go from the standard curve numbers to actual sample values?

I believe I can make the Tecan calculate them, but I'd be just as happy with manual calculations at this point.

Tony Rook
Tony Rook's picture
Kwaraska: Here is a link to

Kwaraska: Here is a link to the Quant-iT Pico Green dsDNA Reagent Kit from Invitrogen. This link takes you to the pdf of this product insert which explains how to generate a standard curve with pico Green and generate quantitative data from your samples using this std curve. Quant-iT PicoGreen dsDNA Reagent and Kits (PDF) Hope this helps. Tony Rook Molecular Biology Moderator