room temperature plasmid DNA storage

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JC in Socal
JC in Socal's picture
room temperature plasmid DNA storage

I was wondering if anyone have any information regarding the kind of material(s) best for storing plasmid DNAs at room temperature besides the eppendorf tubing material. I tried
storing the DNAs in glass vials in presence of both water and glycerol and found the DNAs starting to degrade within 3 weeks at room temperature. Any info or leads on this would be greatly appreciated. Thanks so much!

AUen's picture
I dont have a helpful

I dont have a helpful suggestion...
But I wanted to ask why room temperature and not refrigerator or freezer?

Fraser Moss
Fraser Moss's picture
Biomatrica make a range of

Biomatrica make a range of products for room temperature storage of nucleic acids

for plasmids there is DNA SampleMatrix which preserves and stores genomic and plasmid DNA at room temperature.

Tube and 96-well formats, available as QIAsafe™, can be purchased from Qiagen.

Ivan Delgado
Ivan Delgado's picture
I recently came across Qiagen

I recently came across Qiagen's QIAsafe DNA tubes (and 96-well plates) that do exactly what you are looking for. Take a look here:
The tubes are a bit pricy ($3.40 per tube), but if you go with the plates you are talking less than 50 cents per well.

The FFM's picture





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These technologies were I think originally developed to store DNA/RNA samples in "the field" where scientist do not carry a freezer with them wherever they go, and they don't want to haul around boxes of dry ice.  However the expansion of the technologies into long-term sample storage is a great development that could change for the better how we all store our samples.
Has anyone done the math on the cost of for example 2 years storage of 100 tubes in a freezer vs room temp storage of the same samples in tubes/plates such as these?
With no freezer there is no initial freezer purchase, service/maintenance or electricity bill and no environmental contamination due to coolant disposal etc at the end of the freezer's life.  No freezers may also reduce space requirements for sample storage in a lab.
However, I think even if a financially and/or environmentally contentious lab were to start using room temp storage instead of freezers on a large scale I do not expect that the institution would note the energy and maintenance savings to the individual lab and then return the favor in the form of an overhead reduction.  That said the potential savings are probably more apparent to the management of small biotech operations who are in more direct contact with their research staff.
I think that when marketing to the larger institutions (Big Pharma/Universities) the companies that sell this interesting technology rather than targeting the grass roots scientist who actually use the product day to day, need to aggressively market to the heads of department and campus operations managers who run the academic or pharmaceutical research sites and who charge the overheads to the labs for energy consumption and for the square footage of lab space that they use.   Then any savings made by the labs that adopt the technology could be rewarded by overhead reductions.
Anyone else got an opinion on this?

ikerman's picture
For full disclosure, I am an

For full disclosure, I am an employee at Biomatrica, however this topic speaks directly to something that I'm currently working on and so I thought I would comment.
There are some room-temperature/no-freezer technologies that have been focused on field collection and the such, but Biomatrica's approach has been focused more on laboratory storage, biobanking, and forensics. Based on an internal study that we have done with a couple of major univisities, there is a great potential for cost and environmental savings when using room-temperature based storage instead of freezer based storage.
For only 100 tubes, you're not likely to see much if any benefit because you're not going to eliminate a freezer with that small of a sample. However, when looking at an institute or university-wdie adoption, you could see a savings of $0.05-$0.10 per sample per year. There's obviously a number of factors that go into this calculation, so the savings will vary by case. However, the above numbers are based on a very rigorous model which forecasts 10 years ahead for a major university. If you count the number of DNA samples in your lab and others, you'll see that the cost savings can be quite large. There's also a large reduction in the univerisity's carbon footprint through the use of less energy.
If you'd like more information or would like to discuss this further, please feel free to contact me.