Analysis of simple binding assay

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dimps's picture
Analysis of simple binding assay

Dear all,
Please could someone help, I have conducted a very simple radioligand binding assay using tritinated nms and CHO cells. I used one concentration of ligand and also use a nonradioactive ligand of atropine to assess specific and nonspecific binding. My results are as expected but need some help with analysis. I have told to work out Bmax, kd etc but i thought that you could work out Bmax only when you use varying concentrations of agonist. In my assay this was constant. Can anyone please please remind me of the basics again as all the info on the net refers to bmax and varying concentrations of agonist! thank u ever soo much!