Membrane protein purification

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Kshirsagar D
Kshirsagar D's picture
Membrane protein purification

Dear all,

I want to purify the hydantoinase from Pseudomonas aeruginosa, a membrane protein , it is not stable in any detergent solution , how I should proceed ?

Abhishek Singh
Abhishek Singh's picture
do you need how to lyse cells

do you need how to lyse cells without using detergent. hypotonic buffer and centrfugation might help