oligonucleotide probe best for cDNA library or genomic DNA library

1 post / 0 new
pavitra2009
pavitra2009's picture
oligonucleotide probe best for cDNA library or genomic DNA library

You have an oligonucleotide probe that hybridizes to part of gene A from a eucaryotic cell.  Indicate whether a cDNA library or a genomic DNA library will be more appropriate to use for the following applications.
A.        You want to study the promoter of a gene A.
B.         Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-length sequence of the tRNA.
C.        You discover that Gene A is alternatively spliced and you want to see which predicted alternative splice products are actually produced in a cell.
D.        You want to find both gene A and the genes located near gene A on the chromosome.
E.         You want to express gene A in bacteria to produce lots of protein A.