processing for histology

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aptafreshi
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processing for histology

I was wondering if a cryopreserved tissue in sucrose/formalin could be also used for paraffin embedding, that is, going through series of alcohol after sucrose cryopreservation?

peter36
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We do fat stains ie frozen

We do fat stains ie frozen sections on fixed adrenal glands. These are soaked in 7.5% sucrose for 1hr then frozen at -20c this helps cryostat sectioning. After they are processed the usual way no problems arise except occassionally crystal artifact

aptafreshi
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Dear Peter,

Dear Peter,
Thanks so much for your reply. What I meant is not processing the cryosections but the sucrose embedded tissues kept at 4 degrees for paraffin embedding thereafter.
Looking forward.

All the best
Azita

peter36
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Yes, sorry that is what i

Yes, sorry that is what i meant, we have no problem processing the sucrose embedded tissue.
regards pete

aptafreshi
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Dear Peter,

Dear Peter,
Thanks again. I need to process rat brain soaked in sucrose-formalin 10% for paraffin embedding. Do you have any timing schedule for that? Looking forward.

All the best
Azita

peter36
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I will get the schedule from

I will get the schedule from work will reply in couple days (is saturday now)
pete