is rnase treatment is necessary for purity

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kayani's picture
is rnase treatment is necessary for purity

i have isolated dna from different maize hybrids but the bands are not so much i m thinking about rnase treatment bcz in my bands rna cintaminatin is this will clear the dna bands.
ms student

Rajeshwari patel
Rajeshwari patel's picture
Dear kayani,

Dear kayani,

hows your DNA band it is smear or it is in low quantitity.
I Don;t think your RNA contamination if affectig your DNA quality in the gel. For further confirmation you need to check your 260/280 ratio from that you come to know your DNA quality.