2-Mercaptoethanol

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Felicity Emmanuel
Felicity Emmanuel's picture
2-Mercaptoethanol

Hi, please can anyone advise on how to prepare 20mL of 5mM 2-mercaptoethanol?
I have 25mg bottle of liquid 2-mercaptoethanol from WAKO, JAPAN but I need to prepare 5mM stock for some experiments, problem is aside from the molecular mass which is given on the bottle there is no other indication of what  the concentration of 2-mercaptoethanol in the bottle is. Please can anyone help? I really need to make my 5mM stock solution but don't know what to do.
I'll look forward to your response. Thanks in advance.

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi felicity,here is a link

Hi felicity,
here is a link with the help of which you can get the desired molarity or as you want just enter the values like mass, volume required, concentration which i suppose may be mentioned on the bottle (98-99%) and you will get the result easy...
www.restrictionmapper.org/dilutioncalc9.html

Jason King
Jason King's picture
MW of beta-Me is 78.13

MW of beta-Me is 78.13

You have 25mg

ie.
78.13g => 1 mole
25mg => 0.32 m.moles               (25/78130 x 1mole)

You want a 5mM solution = 5m.moles/L
so the 0.32 m.moles that you have will need to be diluted to a volume of 64mL

                                                          (0.32/5 x 1000mL = 64mL)

Maybe someone can double check that?

Jith sn
Jith sn's picture
Kindly find the link below:

Kindly find the link below:

https://www.e-reagent.com/cgi-bin/gx.cgi/AppLogic+ufg280disp_pr.ufg280disp_Main?now=1251364652720

Catalogue no. 137-06862 , 25 gm beta mercapto ethanol has a concentration corresponds to 14.3 mol/L, i guess u can consider this concentration and diluted down to your required concentration say 5mM.

Felicity Emmanuel
Felicity Emmanuel's picture
 Hello,

 Hello,

Thanks for all your responses to my question , I really appreciate them. if however there are other methods you might want to teach me, please do. Knowledge is never too much!.

Anyway, thanks for your help.