beta mercaptoethanol alternative

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dmm520
dmm520's picture
beta mercaptoethanol alternative

Hello--

I've been trying to research reducing agents for protein work and have found some info on DTT and TCEPversus 2-ME but couldn't really find a concise comparison and clear benefits/disadvantages to using one over the other.  I could not find if there is another agent which shows no absorbance between 260-280 nm...I am wondering if I could get an opinion from someone who's used these things before instead of just the manufacturer's information...

I want to use whatever agent absorbs the least around the A280 of aromatic amino acids...

Thanks!

g a
g a's picture
Hi dmm520

Hi dmm520

Tributylphosphine is used at a maximam conc of 2mM. Its absorption extent in diluted samples are tolerable within the limits of detection in microgram range.