Hello! Good day!
I would like to ask some help.
I am having problems with my experiment.
Last December 2010 and January 2011, I have finished the first phase of the research and that is to optimize HPLC conditions. Also, I have analyzed 25% of the total number of samples to analyze. Now that I am continuing the reaseach, the plan is to finish analyzing the remaining samples but the problem is that I don't have peaks which I had before.
I followed the DNA digestion protocol (Chakrabarty et al., 2003) that I used when I had peaks before but now, there are no peaks.
What could possibly be the problem and your suggested solution to this?
I hope you could help me.
Thank you so much.