DNA methylation of a potential crop in the Philippines

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Maria Mabras
Maria Mabras's picture
DNA methylation of a potential crop in the Philippines

Hello! Good day!

I would like to ask some help.

I am having problems with my experiment. 

Last December 2010 and January 2011, I have finished the first phase of the research and that is to optimize HPLC conditions. Also, I have analyzed 25% of the total number of samples to analyze. Now that I am continuing the reaseach, the plan is to finish analyzing the remaining samples but the problem is that I don't have peaks which I had before.

I followed the DNA digestion protocol (Chakrabarty et al., 2003)  that I used when I had peaks before but now,  there are no peaks. 

What could possibly be the problem and your suggested solution to this? 

I hope you could help me.

Thank you so much. 

Sincerely,
Maria