I would like to ask a question.
Is there a possibility that the enzyme would eventually loose its enzymatic activity after 6 to 8 months even though it has not yet reached its expiration date?
I am thinking that it might be the the reason why I am not getting the peaks I got 6 months ago.
I hope you could help me with this problem. The enzymes I use are nuclease p1 and alkaline phosphatase (along with its buffer).
Thank you very much.