DNA methylation profiling using HPLC analysis

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Maria Mabras
Maria Mabras's picture
DNA methylation profiling using HPLC analysis

Hello!

I would like to ask a question.

Is there a possibility that the enzyme would eventually loose its enzymatic activity after 6 to 8 months even though it has not yet reached its expiration date?

I am thinking that it might be the the reason why I am not getting the peaks I got 6 months ago.

I hope you could help me with this problem. The enzymes I use are nuclease p1 and alkaline phosphatase (along with its buffer).

Thank you very much.

Sincerely,
Maria