flow cytometry of mouse spleen

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Shiang's picture
flow cytometry of mouse spleen

For a recent practical I had to investigate different cell populations in a mouse spleen cell suspension which present MHC class II molecules. 2 antibodies were used-rabbit IG which is specifically anti-mouse Ig, and mouse Ig which specifically binds MHC class II.
my problems are:
what ic 'CV'?
why was i required to add mouse serum and rabbit serum?( not counting spleen cell suspension)
any help would be greatly appreciated.