need help on retrograde labeling

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traceruser's picture
need help on retrograde labeling

Hi All,

I am a graduate student in Boston University. The project I am working on includes retrogradely label mitral cells in the mouse main olfactory bulb. I use CTb and it work well (tracer injection injected to amygdala). I also want distal dendrite being labeled. Because the dendrite is very long and the end of the dendrite is not well labeled. I wonder whether there is better way(different tracers/ high concentration) to back label distal dendrite.

Thank you very much !

Cam ( eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%63%61%6d%6d%79%40%62%75%2e%65%64%75%22%3e%63%61%6d%6d%79%40%62%75%2e%65%64%75%3c%2f%61%3e%27%29%3b')))