Protein Extraction

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Felicity Emmanuel
Felicity Emmanuel's picture
Protein Extraction

Please, could someone help me out with this?.
I would like to extract renin from mouse kidney tissue for western blotting,but I am not really sure about it.
I tried using a general protocol of homogenising and centrifuging in a protease inhibitor buffer however, this isn't specific for my protein of interest (renin)
I would like to know if there is a better or more specific protocol for renin extraction from animal tissues ( especially from Kidney tissue)
Thanks in anticipation of your response.
felicity