I have a question on the T4 polymerase
I found the following phrase in a textbook:
T4 polymerase will either elongate or remove nucleotides and this depends on avaibility.
What does this mean?
Example lets say we have
5' GTACGGAT 3'
3' CATGCCTACGTAC 5'
and we add T4 polymerase and some dNTP's , what will happen then?
I suppose the T4 polymerase will add thise nucleotides ? untill the template is completed?
But what do they then mean with "remove nucleotide" ?
anyone an idea?