REPORTER GENE ASSAY

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ksowmya123
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REPORTER GENE ASSAY

I have a question regarding the luciferase reporter gene assay. I performed transient transfection of MCF-7 cells using CYP1B1 reporter plasmid and exposed the cells to 100nM TCDD and found the increase in activity of CYP1B1 promotor activity when i performed luciferase reporter gene assay,I repeated the same experimental procedure for seven times and i could't get the same results and plzz suggest what could be the posiible reason for my poor results.