I need some help in data interpretation in RP-HPLC. I have made a standard graph of my compound from the peak area of each concentration of the standard. Now I have the sample graphs too. I have determined the concentration of each sample from the standard graph using the equation of the line. My question is that, does the peak area, my injection volume and my actual concentration of the standard correspond with each other? In other words, is it right to determine the concentration of compound in my sample from the equation of the graph? Or is there any other method?
Please reply as soon as possible.