URGENT: composition of Hepes Buffered saline

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Amritha Nair
Amritha Nair's picture
URGENT: composition of Hepes Buffered saline

what is the exact composition of the Hepes Buffered saline reagent??

Amar Annamalai
Amar Annamalai's picture
Hi,

Hi,

Please note the composition of HEPES saline,

HEPES 10mM;
NaCl 151mM;
KCl 4.7mM;
CaCl2 2mM;
MgCl2 1.2mM;
glucose 7.8mM.

The pH of this solution should be adjusted to 7.4 using NaOH.

AMAR

mchinmoyee
mchinmoyee's picture
Kindly follow the link also.

Kindly follow the link also.

http://www.mpbio.com/product_info.php?products_id=15884

Importantly keep in mind phototoxicity of HEPES when exposed to ambient light due to the production of hydrogen peroxide. So its strongly advised to keep any HEPES containing solution in dark.

see theses refs :
* Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. Journal of Immunological Methods. 1987 Oct 23;103(1):145.
* Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.
* Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I., In Vitro Cell Dev Biol. 1985 May;21(5):282-7.