UV Spec Question

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dmm520's picture
UV Spec Question

I'm trying to create a calibration curve for a protein on our uv spec; unfortunately everytime i test the absorbance for the protein i get a different answer...how should i prepare the sample so this does not occur? i have tried samples suspended in DI water and also with DI water+ denaturing with 6 Mguanidine Hcl.
Thanks :)