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soniakhan's picture

can anyone please send me the exact and proved protocol of zymography.i tried to do zymography many times with different protocol but i am not getting the clear band as mentioned in many papers,my protein does not seem to run through the gel,so i get clear zone at the top of gel.
you can send at eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%61%71%75%61%72%69%61%6e%39%31%32%40%67%6d%61%69%6c%2e%63%6f%6d%22%3e%61%71%75%61%72%69%61%6e%39%31%32%40%67%6d%61%69%6c%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))