About pulsing target cells with SEB, has anyone ever tried this?

1 post / 0 new
SnowBall
SnowBall's picture
About pulsing target cells with SEB, has anyone ever tried this?

 I'm wondering if anyone ever try pulsing SEB to CD4+T cells instead of target antigen presenting cells (APCs) for antigen presentation assay. Can I pulse SEB to my sorted CD4+T cells (about 2 hours)  then co-culture them with my APCs to mimic the antigen presentation process? In fact, I've just performed this yesterday.