Does anyone has experience in studying anti-DNA response. I think I have some problem in my protocol. I am using methylated BSA (in PBS) for pre-coating and calf thymus DNA as an antigen (in 1mM Tris, 1mM NaCl, and 1mM EDTA). My blocking buffer is 3% BSA and 0.02% NaN3 in PBS.
I will appreciate any comments.