I am interested in isolating B cells from a PBMC sample using the B Cell Isolation Kit II from Miltenyi Biotec. The source is donor whole blood, I believe. I am wondering whether to use MS Columns with MiniMACs or LS Columns with MidiMACs.
A secondary question would be if I can freeze the B cells, or keep them in culture for a few days. My goal would be to do functional assays on them, and I'd need at least a couple of million B cells per assay.