I have been doing BMDM cultures using L929 conditioned medium for quite some time. Recently few BMBDM batches have been behaving strangely. A population of mcarophages appears black and activated, while others appears round and healthy. Slowly by day 3 many such macrophages begin to appear and by the end of 7 hardly any macrophages appear normal.
I cleaned everything from medium to incubator after I had problems with the first BMDM batch, but the problem still persists. Has anyone faced this problem and found a solution to it? Any suggestions?