Cell survival during starvation

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wellshao
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Cell survival during starvation

Excuse me, I am researching how glucose protects cell from apoptosis? During cell starvation, how can I make cell survive without FBS and glucose? Thank you very much!

Ivan Delgado
Ivan Delgado's picture
 

 
I am not sure if I understand what you are asking, but here are my two cents. When you starve cells (typically to synchronize them), you are only removing the serum, so they should be fine with serum-free media. Of course you do not want to do this for too long or they will die. You can always add some BSA to aid in keeping the cells stable (typically about 0.2%). You should be fine for 24 to 48 hours.

wellshao
wellshao's picture
Ivan wrote:

Ivan wrote:

Thank your nice suggestion. My plan is:
To explore whether glucose can weaken the DNA damage in B cells, I first examine the glucose effect on rescuing the cytotoxicity of etoposide. After stavation, Raji cells(suspension cells) will be cultured in glucose-free medium, treated with or without etoposide and glucose in different time. But how to and how long starve cells, not to harm cells?
Thank again.
I am not sure if I understand what you are asking, but here are my two cents. When you starve cells (typically to synchronize them), you are only removing the serum, so they should be fine with serum-free media. Of course you do not want to do this for too long or they will die. You can always add some BSA to aid in keeping the cells stable (typically about 0.2%). You should be fine for 24 to 48 hours.
Ivan Delgado
Ivan Delgado's picture
 

 
I understand what you are trying to do but honestly I do not have experience growing cells without glucose. I think you may have to do some preliminary experiments to determine just how long your cells will be able to survive without glucose before running the actual experiment (something I would recommend even if growth without glucose was better defined).
 

heehawmcduff
heehawmcduff's picture
Depending on what your

Depending on what your reasons are for serum-starving your cells, you could always use some sort of serum substitute such as Ultroser G (Pall Pharmaceuticals) - this obviously would not help if you are quiescing your cells prior to treatment.
Regards

wellshao
wellshao's picture
Thank you very much!

Thank you very much!
heehawmcduff wrote:

Depending on what your reasons are for serum-starving your cells, you could always use some sort of serum substitute such as Ultroser G (Pall Pharmaceuticals) - this obviously would not help if you are quiescing your cells prior to treatment.
Regards