Dendritic Cell Maturation Factors

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beansta7
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Dendritic Cell Maturation Factors

What are alternative methods to generate human monocyte derived dendritic cells to CD83 expression? Currently we are using GM-CSF and IL-4 5D + LPS 2D.

Li Li
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It seems that you have to use

It seems that you have to use GM-CSF and IL-4 for generating immature DC, but you may use TNFa instead of LPS for DC maturation for CD83 expression.
Li Li

Big E
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What are alternative methods

What are alternative methods to generate human monocyte derived dendritic cells to CD83 expression? Currently we are using GM-CSF and IL-4 5D + LPS 2D.

You can use CpG DNA, cd154 (cd40L), and Light (HVEM-L) as well for maturation. I also recall seeing a paper where you could directly maturate with cd154 that is 4 hrs adherence of monocytes then direct addition of cd154 and the cells will behave as if they are maturated and skip over immature DCs state. Realize though that all these monocyte dervived DCs will have some differences in cell surface changes and functionality depending on the maturation factors utilized.

venky04
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Big E wrote:What are

Big E wrote:

What are alternative methods to generate human monocyte derived dendritic cells to CD83 expression? Currently we are using GM-CSF and IL-4 5D + LPS 2D.

Perhaps the following article may answer your question:

Obermaier B. et al. (2003) Biological Procedures Online, vol. 5 No. 1; pp. 197-203. Oct. 24 issue. Development of a new protocol for 2-day generation of mature DCs from human monocytes.