I routinely use a M&N Nucleospin RNA II kit to make total RNA (which I then use for cDNA and qPCR).
Just recently I did a flow sort of neutrophils to get a million neuts (free of eosinophils) from which I had hoped to make RNA. Amazingly, the spec' suggested that absolutely no RNA had be purified from these million cells.
Neuts are known to be transcriptionally "quiet", but I thought I'd get something out of it.
Does anyone know how many neuts to start with?