IL-2 Dependent NK-92 Cells

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beansta7
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IL-2 Dependent NK-92 Cells

What concentration of Recombinant IL-2 works best for maintaining cell line NK-92 in culture?

Soudabeh
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beansta7 wrote:What

beansta7 wrote:

What concentration of Recombinant IL-2 works best for maintaining cell line NK-92 in culture?

Most companies that provide human recombinant cytokines perform their own protein proliferation assays and give you a range of ED50. The rest is up to you to evaluate . For e.g. If you use Human recombinant IL-2 from R and D Systems, Inc. the ED50 for proliferation is 0.25-0.5 ng/ml. I think you should start with a lower concentration and monitor the amount until you find out the optimum concentration for your cell line.