immuofluorescence on fixed tissue

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nkm
nkm's picture
immuofluorescence on fixed tissue

HI,
I was wondering if anyone had a protocol for immunofluoresence on tisse rather than fixed cells... would it be any different?
also is it better to use parrafin embedded tissue or cryosections?

thanks

Guy Sovak
Guy Sovak's picture
I would prefer using a

I would prefer using a cryosection. The protocol is the same as the regular IHC.
Just be sure that you block non specific 15min and wash well after each step.
You can also embed the tissue in OCT.
Please find a link to an article which describes a protocol for rat liver embeded in OCT
http://www.fasebj.org/cgi/content/full/17/6/660
Guy

nkm
nkm's picture
Thanks so much,

Thanks so much,

just wondering ..do you think the protocol would differ much with lung tissue...
with paraffin embedded is the main issue autofluorescence problems?

thanks again for your help