I have been trying to culture the only mouse dendritic cell line available JAWS II, and was wondering if anyone had experience with that?
I am using the recommended ATCC medium:
alpha-mem with ribonucleosides, deoxyribonucleosides, 4mM Glutamine, 20% FBS 1mM sodium pyruvate and 5ng/ml GM-CSF.
The cells seem to be happy and healthy for about 3 weeks, proliferating at a slow but steady rate. And then they would all die at the same time (even separate plates) one day...
I haven't let them become more than ~60% confluency before splitting and been changing media every 2 days.
It's the third time it has happened to me, and I am not sure what's happening!
Any help/advice would be GREATLY appreciated!