I will do a large scale macrophage isolation in the near future and would like to pool the monocytes from donors (prob.)with different blood types.
I am using the Ficoll and adherent cells protocol. Well theoritically there shouldn't be any problem but I'm worried that my isolation could still have trace of contamination by other cells that may interact.
Before doing a mistake, I would like to know if any of you have had the experience with macrophages from different blood types. Can I just mix them together?
Large scale macrophage isolation from different donors?