Low amount of induced peripheral macrophages

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freshme
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Low amount of induced peripheral macrophages

Hi everybody! I am new in primary macrophage culture. Refering to solutions here, I use 3% thioglycollate broth to induce peripheral macrophages. But  the prepared  3% thio. medium seems inefficient. I injected 2ml medium per day for three days and can just harvest not more than 10-6 cells per mouse afrer 3 days latter. The thio. medium is from Sigma. I don't know if the new prepared thio. medium is so low efficiency in eliciting macs?
Thanks~

In addition, the attachment is a picture of my culture of primary macrophages, can anyone tell me if the cell is the mature macrophage? thanks again!

freshme
freshme's picture
anybody can help me?  the

anybody can help me?  the same problem haunts me.