Hi everybody! I am new in primary macrophage culture. Refering to solutions here, I use 3% thioglycollate broth to induce peripheral macrophages. But the prepared 3% thio. medium seems inefficient. I injected 2ml medium per day for three days and can just harvest not more than 10-6 cells per mouse afrer 3 days latter. The thio. medium is from Sigma. I don't know if the new prepared thio. medium is so low efficiency in eliciting macs?
In addition, the attachment is a picture of my culture of primary macrophages, can anyone tell me if the cell is the mature macrophage? thanks again!