Macrophage again-- please help

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sak
sak's picture
Macrophage again-- please help

Hi.
I'm new to macrophage works, so I have this very basic question.
I figured that people use thioglycollate to eleicit peritoneal macrophages. Are these macrophages activated or are they immature?
Also, where do you get sucrose solution?

Thanks~!!!

Guy Sovak
Guy Sovak's picture
Hi there,

Hi there,
Yes they are activated look at this link.
http://www.jimmunol.org/cgi/content/abstract/128/1/433
As per the Sucrose solution you can prepare it.
Guy

sak
sak's picture
guy wrote:Hi there,

guy wrote:

Hi there,
Yes they are activated look at this link.
http://www.jimmunol.org/cgi/content/abstract/128/1/433
As per the Sucrose solution you can prepare it.
Guy

Thank you so much.
Oh.. I've got another question.
Which thioglycollate should I use?
we have fluid thio medium from Difco for micro work.
Is this O.K., or sould I get "thioglycollate berwer modified" for injection???

samm
samm's picture
sak wrote:

sak wrote:

Thank you so much.
Oh.. I've got another question.
Which thioglycollate should I use?
we have fluid thio medium from Difco for micro work.
Is this O.K., or sould I get "thioglycollate berwer modified" for injection???

Hi! Take a look at the macrophage isolation protocol in this site - you can search for it. That will give you additional details on the procedure.