mitogen for Jurkat cells

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pva
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mitogen for Jurkat cells

I´m trying to stimulate a Jurkat cell line with PMA/IoM and unfortunatelly with no success. After 24 h treatment I have more cells in the test cells  wells ( Cell cultured in RPMI 10% FBS) as in those wells  where the mitogen was added. Could you give me some advice?
Many thanks