I need to count the number of mouse macrophages that ingest rabbit IgG-coated sheep erythrocytes
A few questions if you can help:
How many mice will I need to start with?
How many peritoneal cells do we expect per mouse?
At what speed / temperature / time should I centrifuge the mouse peritoneal cells to isolate the macrophages?
In the phagocytosis assay, the final counting of macrophages with ingested erythrocytes, how do we count the cells? From the whole cover glass or how many random areas
( and if you could help on a part time basis, I'd appreciate it.)