I am working with Biogel-elicited macrophages and the last few months I have observed greatly reduced macrophage viability (even with 15h of culture, while before they were easily lasting for 3 days) and increased neutrophilia in my PECs. The viability of thioglycollate macrophages is not affected. And no obvious symptom is observed in my C57BL/6J.
I changed everything, including my Biogel Batch but I still observe macrophage death and neutrophilia (about 50% of my PECs!). I am thinking it is a virus infection affecting specifically the biogel-type of macrophages as I can not classify all macrophage-elicitation methods as one. But viruses like MHV affect both Biogel and thioglycollate. I don't want to swtich in the middle of my experiments to another cell type... Any ideas about what could be causing this??