I want to know where's a scientific group who isolate B cells from human blood.
My concern is that if I want to get mAb gene for cloning, I should find a way to isolate single B cell and conduct RT-PCR.
Cell based On-chip microarray format will be the best if there is.
But there was actually a group in japan that published a paper in NATURE.
Except that group, hope to know if there's anyone else.
1. those who can screen B cells high-thoughput.
2. And they should pick up just a single Antibody Secreting B cells to do RT-PCR
3. and then They should perform Cloing to have a antigen-specific antibody as a mount of human mAb.
FACS sorting or anyother multi-well plate format will be also fine.
Can such there be????