i am quite new to this topic and i could really need some help.
What i want to do is inject thioglycolate (i got the brewer's medium) i.p and extract macrophages 3-4 days later to isolate RNA for receptor studies. So here are my questions:
1.How much TG will i have to inject? (I have read about ranges from 0.1 to 3ml)
2.How do i extract the macrophages? When we tried it once in our lab we injected ice cold cell culture medium into the peritoneum but we couldnt get it back out, because the bowels were always aspirated.
I would be very thankful if anyone could help me out with a protocol!