Total Rhesus Splenocyte number

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Amtekoth
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Total Rhesus Splenocyte number

 Hi,
Next week I'm getting a Rhesus macaque spleen and I need to have a rough idea how many splenocytes I can expect to get out of it so that I can plan my experiments and set up tubes for freezing.
Thanks,
Ed

Satinder
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dear Amtekoth

dear Amtekoth
I did tried some web searching for the topic but couldnot find any relevant information. However the total volumetric capacity is what i could find. It is approximately 0.3 gallons. May be that is of certain help for you.
If you dont get any help in this regards I would suggest to estimate arbitarary volume of monkey spleen and use it to calculate splenocyte number by multiplying it with the size fold difference as comapred to mouse spleen...

Satinder
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Hi

Hi
I did find an article but my institutional library doesnt have subscription to the same so its not opening over here. Perhaps You can access it. The article shows CBC data of rhesus macaques spleen
Flow cytometric characterization of T lymphocyte subsets in the peripheral blood of Chinese rhesus macaques: normal range, age- and sex-related differences.

by: CL Qiu, H Zhao, GB Yang, Q Liu, Y Shao

Veterinary immunology and immunopathology, Vol. 124, No. 3-4. (15 August 2008), pp. 313-321.
 
Available data on the normal levels of white blood cell populations in healthy rhesus macaques (Macaca mulatta) originated and living in China is scanty. To obtain such data, blood samples from 150 Chinese rhesus macaques were collected and the normal range of white blood cells and their subsets were analyzed according to age and sex by flow cytometry. CBC data showed that the count of total white blood cells and lymphocytes decreased with age. Phenotypic analysis of CD4 and CD8 expression on CD3+ T lymphocytes showed that the percentage of CD4+ T cells (51.4+/-9.6%), CD4-CD8- T cells (8.5+/-4.1%) and the ratio of CD4+ T to CD8+ T cells (1.26+/-0.55) decreased with age; and the percentage of CD8+ T cells (42.0+/-9.7%), CD4+CD8+ T cells (1.3+/-0.9%) and CD3+ lymphocytes (55.3+/-13.3%) increased with age. However, no statistically significant difference was observed between the male and female groups in most parameters in these monkeys except for the percentage of CD4+CD8+ T cells. This study provided basic information about blood cell count and T lymphocyte subsets in Chinese rhesus macaques. It may be useful for comparative studies using Indian and Chinese rhesus macaques.
 

Amtekoth
Amtekoth's picture
 Thanks for the flow

 Thanks for the flow cytometry/T-Cell reference, Satinder.  Unfortunately, it did not contain the information on splenocytes that I need.  I'll keep searching.