Which induces a stronger primary immune response?

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wellshao
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Which induces a stronger primary immune response?

I plan to use KLH or NP-KLH to immunize mice with i.p.
10 days after immunization, spleen was isolated for sorting germinal center B cells later.
Could you tell me which induces a stronger primary immune response  KLH or NP-KLH?, or there is no difference?
Thank you very much!

g a
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NP KLH is better than KLH

NP KLH is better than KLH alone as peptides are conjugated and presented as a single unit with it.

wellshao
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Thank you very much! I am

Thank you very much! I am still strange what is the different immune response for NP-KLH,NP-CGG,NP-OVA ,SRBC and others after primary immunization?
Thank you again.

Satinder
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Immune response is totaly

Immune response is totaly dependent upon the phylogenetic distance as well as complexity of the antigen. That is why there is variable degree of immunogenicity as achieved by the different immunogens.

wellshao
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I want to see the change of

I want to see the change of germinal cencer from mice spleen or Lymph node. I immunized with KLH, but some one tell me KLH is weak to activate primary immune response. Do you think so?

Satinder
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KLH is keyhole limpet

KLH is keyhole limpet hemocyanin.
Its a mollusc protein and is phylogenetically VERY different from the rodent counterpart. Its expected to be strongly immunogenic. Further more KLH has a very high Mol Wt 4.5 X 10e5 ~ 1.3 X 10e7. Which further adds to its immunogenicity.
Still, If you are not getting better responses then you can switch over to some other immunogens like ovalbumin, tuberculosis attenuated bacteria etc.

wellshao
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Thank you very much!

Thank you very much!